The ompATb gene was amplified from pML588 by PCR using the primers 960 and 962 (Song et al., 2008). This fragment was digested with NcoI and SwaI and ligated into similarly digested pET28b+ (Novagen), resulting in pML591. Overexpression of ompATb in E. coli For protein expression and purification, a 1 L culture was grown to OD600 of 0.6-1.0, and induced with 0.5 mM isopropyl-beta-D-thiogalactopyra
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