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Al needle [12]. Inflammation appeared within 2 h of the injection and peaked between 6?4 h. PWL was tested with a previously described method [12,13]. Each rat was placed under an inverted clear plastic chamber on the glass surface of the Paw Thermal Stimulator System (UCSD, San Diego) and allowed to acclimatize for 30 min before the test. A radiant heat stimulus was applied to the plantar surface

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