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E previously showed that the KXD/E motif binds COPI coatomers to retain AtEMP12 in the Golgi of Arabidopsis (Gao et al., 2012). To examine whether the KXD/E motif has similar COPI-binding ability in other organisms, we used synthetic peptides corresponding to the AtEMP12 CT as bait to perform pull-down assays using proteins from yeast cell extracts followed by SDS AGE separation and tandem mass sp
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Samples that had multiple respiratory viruses detection (average = 31.27). Of the 762 samples with visible mould, 529 (69.4 ) were positive for ERV3, which was significantly lower than rates in samples without visible mould (84.0 ; crude OR = 0.35, 95 CI 0.28-0.43). The proportion of samples with visible mould and positive respiratory virus testing (178/762; 23.4 ) was significantly lower than th
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This shows the importance of measuring human DNA as a marker for epithelial cells in swab samples, which if tested and monitored in real time during the study, can identify problems associated with collection that can be addressed quickly. This is illustrated in the current study when a sudden increase in ERV3 negative samples was observed. Parents were contacted and reminded about sample collecti
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Standard deviation) time from collection until being frozen in the laboratory for samples with no observed mould was 2.9 (3.0) days. In comparison for low level mould it was 4.9 (3.6) days (crude mean difference compared with no mould; 95 confidence interval (CI) = 1.7; 1.4 ?2.1 days), for medium level mould it was 7.4 (4.9) days (3.9; 3.4 ?4.3), and for high level mould 11.4 (10.7) days (7.1; 6.
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Was especially evident during the warm, humid spring and summer months, which leads us to speculate that fungal contamination occurred during sample collection and was influenced by the aforementioned environmental factors. Unfortunately, we could not explore this further as it was beyond the scope of the present study. In addition, while mould growth proved to be an issue in the subtropical clima
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Ith COPI (Sato et al., 2001). Truncation of the C-terminal 10 residues of Rer1p (Rer1p-10) results in mislocalization of Rer1p to the vacuole (Sato et al., 2001). As shown in Figure 6D, after attachment of the KXD/E motif to the C-terminus of the mislocalized Rer1p-10, the GFP-Rer1p-10-RNIKCD recovered to punctate GFP signals in the wild-type strain RSY255 under permissive and restrictive temperat
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