1
Mals produced antibodies which recognized gHt and gL on a Western blot of a denaturing gel (Fig. 5A). On a Western blot of a nondenaturing (native) gel (6), these antibodies also recognized higherTABLE 1. HSV neutralizing activity of sera from rabbits immunized with gHt-gLVirus neutralization titer (50 ) Adjuvant Rabbit Entry assay,a HSV-1(hrR3) Plaque assayb HSV-1(KOS) HSV-2(333)Freund's R136 R13
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Tion (Fig. 4A). As expected from previous studies (57), gC-1t did not block virus entry and served as a negative control for the assay. Fifty percent inhibition of virus entry was observed at 50 nM gD1(306t), a result similar to that obtained in a 50 inhibition of plaque formation assay (43). In contrast, gHt-gL did not inhibit virus entry even at protein concentrations as high as 350 nM (50 ng/
1
Lock HSV infection (31, 42, 43). This is due to the interaction of gDt with cellular receptors such as herpesvirus entry mediator (HVEM) (40, 60), making them unavailable to bind to gD in the virion. In contrast, soluble forms of gC-1 (gC-1t) do not block plaque formation by HSV (57). We recently showed that gC-1t, gB-1t, and gHt-gL did not bind directly to HVEM (60). Here we asked whether soluble
1
Lock HSV infection (31, 42, 43). This is due to the interaction of gDt with cellular receptors such as herpesvirus entry mediator (HVEM) (40, 60), making them unavailable to bind to gD in the virion. In contrast, soluble forms of gC-1 (gC-1t) do not block plaque formation by HSV (57). We recently showed that gC-1t, gB-1t, and gHt-gL did not bind directly to HVEM (60). Here we asked whether soluble
1
Lock HSV infection (31, 42, 43). This is due to the interaction of gDt with cellular receptors such as herpesvirus entry mediator (HVEM) (40, 60), making them unavailable to bind to gD in the virion. In contrast, soluble forms of gC-1 (gC-1t) do not block plaque formation by HSV (57). We recently showed that gC-1t, gB-1t, and gHt-gL did not bind directly to HVEM (60). Here we asked whether soluble
1
Lymorphisms and rare CNVs. Nature Genetics 2008, 40:1253-1260. 24. Nannya Y, Sanada M, Nakazaki K, Hosoya N, Wang L, Hangaishi A, Kurokawa M, Chiba S, Bailey DK, Kennedy GC, Ogawa S: A Robust Algorithm for Copy Number Detection Using High-Density Oligonucleotide Single Nucleotide Polymorphism Genotyping Arrays. Cancer Res 2005, 65:6071-6079. 25. Li C, Wong WH: DNA-Chip Analyzer (dChip). In The ana
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Ntrifugation at 30,000 g for 5 h through a 10 -30 -60 sucrose-PBS step gradient. The virus band located at the 30 -60 sucrose interface was collected, titered, and stored at 80 . Soluble HSV glycoproteins and infected cell extracts used. Soluble gD1(306t) was produced in baculovirus-infected Sf9 cells and was purified as previously described (52). Cytoplasmic extracts of HSV-1(NS) (16)- or HSV-2
1
Lymorphisms and rare CNVs. Nature Genetics 2008, 40:1253-1260. 24. Nannya Y, Sanada M, Nakazaki K, Hosoya N, Wang L, Hangaishi A, Kurokawa M, Chiba S, Bailey DK, Kennedy GC, Ogawa S: A Robust Algorithm for Copy Number Detection Using High-Density Oligonucleotide Single Nucleotide Polymorphism Genotyping Arrays. Cancer Res 2005, 65:6071-6079. 25. Li C, Wong WH: DNA-Chip Analyzer (dChip). In The ana
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