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Cence method. Sections were blocked in PBS with 10 normal donkey serum for 60 min, incubated overnight at room temperature with a mixture of goat polyclonal antibody against CRH (Santa Cruz Biotechnology, Inc., 1:100) and rabbit polyclonal antibody against P-NR1 (Upstate, Serine 896, 1:100). After three 10-minute washings in PBS, sections were incubated in a mixture of CY2conjugated donkey anti-r
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Cence method. Sections were blocked in PBS with 10 normal donkey serum for 60 min, incubated overnight at room temperature with a mixture of goat polyclonal antibody against CRH (Santa Cruz Biotechnology, Inc., 1:100) and rabbit polyclonal antibody against P-NR1 (Upstate, Serine 896, 1:100). After three 10-minute washings in PBS, sections were incubated in a mixture of CY2conjugated donkey anti-r
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Le bar = 10 m.lator. Here, we demonstrated that in the adult brain of rats, macaques, and humans YB-1 is predominantly expressed in neurons. So far postnatal expression in neurons has only been described in mouse and rat brain based on Western Blot analysis of brain tissue extracts or on studies using primary cultures of hippocampal neurons [24,25]. Funakoshi et al. [25] described a decline of mou
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Le bar = 10 m.lator. Here, we demonstrated that in the adult brain of rats, macaques, and humans YB-1 is predominantly expressed in neurons. So far postnatal expression in neurons has only been described in mouse and rat brain based on Western Blot analysis of brain tissue extracts or on studies using primary cultures of hippocampal neurons [24,25]. Funakoshi et al. [25] described a decline of mou
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supervision was uncomfortable for patients and nurses. You should just say, `I'm just out here and I will veri
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In did not significantly change hind paw edema compared with that of sham EA rats plus vehicle, indicating that astressin had little effect on edema. Figure 5 shows the effects of astressin on EA-produced anti-hyperalgesia. PWL of the injected paw was significantly shorter compared to baseline while contralateral PWL did not change (data not shown). EA treatment significantly (P
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