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Run in duplicate, with an average intra-assay coefficient of variation of 5.2 . Samples that read as below the detection limit of 0.0001 mg/L, above 10 mg/L, or with coefficients of variation over 20 were rerun. The average intraassay coefficient of variation for the low control was 2.4 and for the high control was 3.1 . The average inter-assay coefficient of variation for the low controlwas 23.
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Ments on the manuscript and statistical discussion. Thanks to B Cox for assistance in primer testing. Thanks to anonymous reviewers for comments on the manuscript. Author details 1 Centre for Biomedical Research, Department of Biology, University of Victoria, Victoria, BC V8W 3N5, Canada. 2Aquatic Genomics Research Center, National Research Institute of Fisheries Science, Fisheries Research Agency
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Ping physiological categories in ways that optimize fitness [5, 38]. The adolescent transition is a particularly useful time to consider energetic dynamics, as during puberty investment in linear growth is first increased (the adolescent growth spurt) and then terminated. Meanwhile, investment in reproduction, manifested in females as the development of primary and secondary sexual characteristics
1
Ping physiological categories in ways that optimize fitness [5, 38]. The adolescent transition is a particularly useful time to consider energetic dynamics, as during puberty investment in linear growth is first increased (the adolescent growth spurt) and then terminated. Meanwhile, investment in reproduction, manifested in females as the development of primary and secondary sexual characteristics
1
Ed and sections were stained. (A) Photomicrographs of staining for VWF after 1 h. (B) Quantitation or VWF release after 1 h (n 8 SD; *, P 0.04). (C) Photomicrographs of staining for MPO in dermal vessels after 1 h. Clusters of cells in small vessels stain for MPO in antibody-treated grafts. (D) Quantitation of MPO-positive cells after 1 h (n 8 SD; *, P 0.03). (E) Quantitation of VWF release after
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