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Ith COPI (Sato et al., 2001). Truncation of the C-terminal 10 residues of Rer1p (Rer1p-10) results in mislocalization of Rer1p to the vacuole (Sato et al., 2001). As shown in Figure 6D, after attachment of the KXD/E motif to the C-terminus of the mislocalized Rer1p-10, the GFP-Rer1p-10-RNIKCD recovered to punctate GFP signals in the wild-type strain RSY255 under permissive and restrictive temperat
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Ith COPI (Sato et al., 2001). Truncation of the C-terminal 10 residues of Rer1p (Rer1p-10) results in mislocalization of Rer1p to the vacuole (Sato et al., 2001). As shown in Figure 6D, after attachment of the KXD/E motif to the C-terminus of the mislocalized Rer1p-10, the GFP-Rer1p-10-RNIKCD recovered to punctate GFP signals in the wild-type strain RSY255 under permissive and restrictive temperat
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In Arabidopsis protoplasts and transgenic plant as assessed by Western blot analysis and reverse transcription-PCR (RT-PCR) detection, respectively (Supplemental Figure S6). After DEX induction, the DEX inducible -COP RNAi seedlings were found to be lethal, proving the essential function of COPI in plant growth and development (Supplemental Figure S6). Transgenic Arabidopsis plants expressing AtEM
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Was especially evident during the warm, humid spring and summer months, which leads us to speculate that fungal contamination occurred during sample collection and was influenced by the aforementioned environmental factors. Unfortunately, we could not explore this further as it was beyond the scope of the present study. In addition, while mould growth proved to be an issue in the subtropical clima
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Standard deviation) time from collection until being frozen in the laboratory for samples with no observed mould was 2.9 (3.0) days. In comparison for low level mould it was 4.9 (3.6) days (crude mean difference compared with no mould; 95 confidence interval (CI) = 1.7; 1.4 ?2.1 days), for medium level mould it was 7.4 (4.9) days (3.9; 3.4 ?4.3), and for high level mould 11.4 (10.7) days (7.1; 6.
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